Sputum sample strorage Connecting our members with provide a sputum sample, before taking antibiotics, have looked on you tube, and tried taking deep breaths...
Sputum Collection for Tuberculosis (TB) Testing. I am suffering from pulmonary TB. My doctor suggested a sputum test. I have a container for collecting sputum sample. Two days ago when I tired to collect a sample, only saliva came out in the container, but there was no sputum. Today while coughing I felt sputum in my mouth and I spitted in the same container . Now my concern is, will the sputum sample be useful even …, Sputum. Patients with dentures should remove those and all patients should rinse their mouths with water. The specimen should be collected directly into the container. Screw the lid back on the container and make sure that it does not leak. It might be necessary to seal the container with sticky tape to prevent any leakage..
Method. Description. Advantage. Disadvantage. Spontaneous sputum sample. Patient coughs up sputum into a sterile container • Inexpensive • Easy to do • Patient may not be able to cough up sputum without assistance or may spit up saliva instead • Health-care worker has to coach and supervise the patient when collecting sputum Sputum Induction. Patient inhales a saline mist … A sputum fungal smear is a laboratory test that looks for fungus in a sputum sample. Sputum is the material that comes up from air passages when you cough deeply. Sputum fungal smear : MedlinePlus Medical Encyclopedia
The first step in the analysis of a fresh sputum sample is a Gram stain to identify the general type of bacteria that may be present and to determine sample adequacy. If a sample contains a significant number of normal cells that line the mouth (squamous epithelial cells), then the sample is not generally considered adequate for culture and a If the sputum sample is obtained in the evening the sample should be kept in the fridge overnight to try and keep the sample fresh. Taking a sputum sample at times when you do not have an infection e.g. when you attend the clinic for a regular appointment, is also helpful for deciding your long term treatment.
Helpful, trusted answers from doctors: Dr. Singh on how long can sputum sample be saved before tested: With some tests, up to 14 days at room temp. http://www Sputum is mucus and is the name used for the coughed-up material from the lower airways (trachea and bronchi). In medicine, sputum samples are usually used for naked eye exam, microbiological investigations of respiratory infections, and cytological investigations of respiratory systems. It is critical that the patient not give a specimen that
Oct 22, 2019В В· Sputum is usually a combination of saliva and mucus that forms in a person's lungs. To collect a sputum sample, doctors generally have the patient cough deeply and spit into a cup. There are often some precautions taken to avoid any contamination of the sample, and sometimes the patient has to drink extra fluid to increase the quantity of the lung secretion. You will need to collect a sample of your sputum to send to a laboratory for testing. The night before the test, drink lots of fluids, such as water or tea. This will help your body make more
Processing of sputum samples. Each sputum sample was split into two equal parts that were randomly allocated; one part was allocated to the standard method in which a nonrefrigerated centrifuge (Haereaus megafuge 1.0) was used, and the other was allocated to the modified method in which a refrigerated centrifuge (Beckman J-6B) was used. Gastric samples cannot be accepted unless they have been adjusted to neutral pH. SPUTUM. Collect sputum using a sputum collection kit. Remove and cap the conical tube. Seal tightly to leakage and send the conical tube to the laboratory. A first morning specimen consisting prevent of 5-10 mL of sputum is preferred. Do not send saliva.
Helpful, trusted answers from doctors: Dr. Singh on how long can sputum sample be saved before tested: With some tests, up to 14 days at room temp. http://www Processing of sputum samples. Each sputum sample was split into two equal parts that were randomly allocated; one part was allocated to the standard method in which a nonrefrigerated centrifuge (Haereaus megafuge 1.0) was used, and the other was allocated to the modified method in which a refrigerated centrifuge (Beckman J-6B) was used.
May 01, 2011В В· Can sputum be refrigerated? Question. It is common practice now to allow refrigeration of sputum samples prior to processing, in some cases up to 24 hours? Our lab does not allow sputum samples to be refrigerated because of the possibility of loss of possible Neisseria and Haemophilus species. Is refrigerating sputum samples acceptable practice? Helpful, trusted answers from doctors: Dr. Singh on how long can sputum sample be saved before tested: With some tests, up to 14 days at room temp. http://www
Processing of sputum samples. Each sputum sample was split into two equal parts that were randomly allocated; one part was allocated to the standard method in which a nonrefrigerated centrifuge (Haereaus megafuge 1.0) was used, and the other was allocated to the modified method in which a refrigerated centrifuge (Beckman J-6B) was used. Sep 11, 2017В В· Sputum samples. Sputum samples can be obtained using a non-invasive or invasive method and ideally should be collected before antibiotics are started. Invasive methods include oropharyngeal or endotracheal suctioning; these are used with patients who are intubated. A sputum trap is connected to the suction catheter to collect the sputum (Fig 1) (Brekle, 2017).
All Answers ( 15) It is recommended to refrigerate (do not freeze) sputum samples as soon as possible after collection to reduce multiplication of other bacteria (fast growers). The yield starts reducing by ten folds at 2-80c after 7 days. The earlier you process after collection the better. Dec 04, 2012 · Given the sensitivity of sputum culture especially with liquid systems (MGIT) over smear microscopy, one would imagine the incremental diagnostic yield of the EM sputum sample to be negligible; however, this is contrary to our findings which are in agreement with the previous studies by either microscopy or culture [7, 18–20].
The first step in the analysis of a fresh sputum sample is a Gram stain to identify the general type of bacteria that may be present and to determine sample adequacy. If a sample contains a significant number of normal cells that line the mouth (squamous epithelial cells), then the sample is not generally considered adequate for culture and a Your doctor will probably need about 1 teaspoon (5 milliliters) of sputum to run the test. Try to spit out as much sputum and as little saliva as you can. There are no known risks to the test.
Oct 22, 2019В В· Sputum is usually a combination of saliva and mucus that forms in a person's lungs. To collect a sputum sample, doctors generally have the patient cough deeply and spit into a cup. There are often some precautions taken to avoid any contamination of the sample, and sometimes the patient has to drink extra fluid to increase the quantity of the lung secretion. Sep 11, 2017В В· Sputum samples. Sputum samples can be obtained using a non-invasive or invasive method and ideally should be collected before antibiotics are started. Invasive methods include oropharyngeal or endotracheal suctioning; these are used with patients who are intubated. A sputum trap is connected to the suction catheter to collect the sputum (Fig 1) (Brekle, 2017).
How much time can you wait to process a sputum sample for. Oct 27, 2011В В· sputum can be stored at refrigerator temperature for five days, AFB remains in the sputum sample and DSSM can be done even after 3 days at room temperature but the odor becomes foul because of putrefaction., Nov 02, 2019В В· The results of a sputum test can take anywhere from a few hours to a few days, depending on what is found. Culture results for bacteria can be obtained rapidly, and usually do not take longer than a day, while the presence of fungus or mold can take as long as a few weeks to obtain because they.
CYTOLOGY--Specimen Collection Guidelines 2-24-10 pdf. Mar 12, 2018В В· Sputum collects in the lower parts of your lungs and bronchi, which are the tube-like pathways that air moves through to reach your lungs. Symptoms that may indicate the need for a sputum culture test include: cough. a fever or chills. fatigue. muscle aches. breathing difficulties. chest pain. https://en.m.wikipedia.org/wiki/Wikipedia:Reference_desk/Archives/Science/2009_February_18 Children and women tend to swallow sputum. During sleep, there may be a tendency to swallow sputum. The object of the gastric lavage is to collect the accumulated sputum from the stomach. To do it right, you should awaken the patient in the morning and so a gastric lavage before anyone in the room has breakfast delivered..
Helpful, trusted answers from doctors: Dr. Singh on how long can sputum sample be saved before tested: With some tests, up to 14 days at room temp. http://www Do this until there is enough sputum to cover the bottom of the bottle. Screw the cap on the sample bottle tightly so it does not leak. Write the date and time you collected the sputum on the label of the sample bottle. Seal the sample bottle in the plastic bag it came with. Do not put more than one sample bottle in each plastic bag.
Can I put sputum sample in fridge? The advice here is fresh as possible, on the morning of handing it in for example, huff x. Content on HealthUnlocked does not replace the relationship between you and doctors or other healthcare professionals nor the advice you receive from them. Children and women tend to swallow sputum. During sleep, there may be a tendency to swallow sputum. The object of the gastric lavage is to collect the accumulated sputum from the stomach. To do it right, you should awaken the patient in the morning and so a gastric lavage before anyone in the room has breakfast delivered.
Oct 27, 2011В В· sputum can be stored at refrigerator temperature for five days, AFB remains in the sputum sample and DSSM can be done even after 3 days at room temperature but the odor becomes foul because of putrefaction. Gastric samples cannot be accepted unless they have been adjusted to neutral pH. SPUTUM. Collect sputum using a sputum collection kit. Remove and cap the conical tube. Seal tightly to leakage and send the conical tube to the laboratory. A first morning specimen consisting prevent of 5-10 mL of sputum is preferred. Do not send saliva.
*don't use more than 20% propylene glycol with water when inducing a sputum specimen in someone suspected of having tuberculosis; a higher concentration of propylene glycol inhibits growth of the pathogen and causes erroneous test results *use 10% to 20% acetylcysteine with water or sodium chloride if propylene glycol isn't available Dec 04, 2012 · Given the sensitivity of sputum culture especially with liquid systems (MGIT) over smear microscopy, one would imagine the incremental diagnostic yield of the EM sputum sample to be negligible; however, this is contrary to our findings which are in agreement with the previous studies by either microscopy or culture [7, 18–20].
Children and women tend to swallow sputum. During sleep, there may be a tendency to swallow sputum. The object of the gastric lavage is to collect the accumulated sputum from the stomach. To do it right, you should awaken the patient in the morning and so a gastric lavage before anyone in the room has breakfast delivered. Sputum Collection Instructions • Sputum is the mucous or phlegm coughed up from your lungs.It is not saliva or mucous from the back of the throat. • Sputum specimens are best if coughed up first thing in the morning, after you have been sleeping at night.
Your doctor will probably need about 1 teaspoon (5 milliliters) of sputum to run the test. Try to spit out as much sputum and as little saliva as you can. There are no known risks to the test. Mar 12, 2018В В· Sputum collects in the lower parts of your lungs and bronchi, which are the tube-like pathways that air moves through to reach your lungs. Symptoms that may indicate the need for a sputum culture test include: cough. a fever or chills. fatigue. muscle aches. breathing difficulties. chest pain.
Sputum. Patients with dentures should remove those and all patients should rinse their mouths with water. The specimen should be collected directly into the container. Screw the lid back on the container and make sure that it does not leak. It might be necessary to seal the container with sticky tape to prevent any leakage. I am suffering from pulmonary TB. My doctor suggested a sputum test. I have a container for collecting sputum sample. Two days ago when I tired to collect a sample, only saliva came out in the container, but there was no sputum. Today while coughing I felt sputum in my mouth and I spitted in the same container . Now my concern is, will the sputum sample be useful even …
Oct 27, 2011 · sputum can be stored at refrigerator temperature for five days, AFB remains in the sputum sample and DSSM can be done even after 3 days at room temperature but the odor becomes foul because of putrefaction. Sputum cups with a fixative are available in the Cytology Lab and Central Lab. Sputum specimens received without fixative will be rejected. Instruction to Patients for Sputum Specimen Collection 1. Label the sputum cup with patient’s name, hospital number and date of collection. 2. An early morning cough specimen gives the best yield of cells.
The first step in the analysis of a fresh sputum sample is a Gram stain to identify the general type of bacteria that may be present and to determine sample adequacy. If a sample contains a significant number of normal cells that line the mouth (squamous epithelial cells), then the sample is not generally considered adequate for culture and a Sputum Collection Instructions • Sputum is the mucous or phlegm coughed up from your lungs.It is not saliva or mucous from the back of the throat. • Sputum specimens are best if coughed up first thing in the morning, after you have been sleeping at night.
Aug 13, 2017В В· Sputum is a type of thick mucus that is produced by the lungs when they are diseased or damaged. It is a substance known as phlegm, and it can be an indicator of many respiratory infections. This *don't use more than 20% propylene glycol with water when inducing a sputum specimen in someone suspected of having tuberculosis; a higher concentration of propylene glycol inhibits growth of the pathogen and causes erroneous test results *use 10% to 20% acetylcysteine with water or sodium chloride if propylene glycol isn't available
Your doctor will probably need about 1 teaspoon (5 milliliters) of sputum to run the test. Try to spit out as much sputum and as little saliva as you can. There are no known risks to the test. Processing of sputum samples. Each sputum sample was split into two equal parts that were randomly allocated; one part was allocated to the standard method in which a nonrefrigerated centrifuge (Haereaus megafuge 1.0) was used, and the other was allocated to the modified method in which a refrigerated centrifuge (Beckman J-6B) was used.
Sputum Collection for Tuberculosis (TB) Testing. Do this until there is enough sputum to cover the bottom of the bottle. Screw the cap on the sample bottle tightly so it does not leak. Write the date and time you collected the sputum on the label of the sample bottle. Seal the sample bottle in the plastic bag it came with. Do not put more than one sample bottle in each plastic bag., Children and women tend to swallow sputum. During sleep, there may be a tendency to swallow sputum. The object of the gastric lavage is to collect the accumulated sputum from the stomach. To do it right, you should awaken the patient in the morning and so a gastric lavage before anyone in the room has breakfast delivered..
Can I put sputum sample in fridge? British Lung Foun.... You will need to collect a sample of your sputum to send to a laboratory for testing. The night before the test, drink lots of fluids, such as water or tea. This will help your body make more, You will need to collect a sample of your sputum to send to a laboratory for testing. The night before the test, drink lots of fluids, such as water or tea. This will help your body make more.
A sputum culture is a test to find germs (such as TB bacteria) that can cause an infection. A sample of sputum is added to a substance that promotes the growth of bacteria. If no bacteria grow, the culture is negative. If bacteria grow, the culture is positive. If TB bacteria grow, then the person has tuberculosis. Sep 11, 2017В В· Sputum samples. Sputum samples can be obtained using a non-invasive or invasive method and ideally should be collected before antibiotics are started. Invasive methods include oropharyngeal or endotracheal suctioning; these are used with patients who are intubated. A sputum trap is connected to the suction catheter to collect the sputum (Fig 1) (Brekle, 2017).
May 01, 2011 · Can sputum be refrigerated? Question. It is common practice now to allow refrigeration of sputum samples prior to processing, in some cases up to 24 hours? Our lab does not allow sputum samples to be refrigerated because of the possibility of loss of possible Neisseria and Haemophilus species. Is refrigerating sputum samples acceptable practice? Apr 23, 2008 · How Long Can I Keep A Urine Specimen Before I Take It To The Dr.and Does It Need To Be Refrigerated? I get back pain and then it will stop when I …
All Answers ( 15) It is recommended to refrigerate (do not freeze) sputum samples as soon as possible after collection to reduce multiplication of other bacteria (fast growers). The yield starts reducing by ten folds at 2-80c after 7 days. The earlier you process after collection the better. provide a sputum sample, before taking antibiotics, have looked on you tube, and tried taking deep breaths...
Your doctor will probably need about 1 teaspoon (5 milliliters) of sputum to run the test. Try to spit out as much sputum and as little saliva as you can. There are no known risks to the test. May 01, 2011В В· Can sputum be refrigerated? Question. It is common practice now to allow refrigeration of sputum samples prior to processing, in some cases up to 24 hours? Our lab does not allow sputum samples to be refrigerated because of the possibility of loss of possible Neisseria and Haemophilus species. Is refrigerating sputum samples acceptable practice?
provide a sputum sample, before taking antibiotics, have looked on you tube, and tried taking deep breaths... May 01, 2011В В· Can sputum be refrigerated? Question. It is common practice now to allow refrigeration of sputum samples prior to processing, in some cases up to 24 hours? Our lab does not allow sputum samples to be refrigerated because of the possibility of loss of possible Neisseria and Haemophilus species. Is refrigerating sputum samples acceptable practice?
The test is done with a sputum sample from deep inside your lungs. You may need to rinse your mouth with water first, then cough up enough mucus for a culture sample to be taken. If you can't cough up enough fluid, your healthcare provider may use a bronchoscope to get a sample … A sputum culture is a test to find germs (such as TB bacteria) that can cause an infection. A sample of sputum is added to a substance that promotes the growth of bacteria. If no bacteria grow, the culture is negative. If bacteria grow, the culture is positive. If TB bacteria grow, then the person has tuberculosis.
Children and women tend to swallow sputum. During sleep, there may be a tendency to swallow sputum. The object of the gastric lavage is to collect the accumulated sputum from the stomach. To do it right, you should awaken the patient in the morning and so a gastric lavage before anyone in the room has breakfast delivered. Sputum is mucus and is the name used for the coughed-up material from the lower airways (trachea and bronchi). In medicine, sputum samples are usually used for naked eye exam, microbiological investigations of respiratory infections, and cytological investigations of respiratory systems. It is critical that the patient not give a specimen that
Oct 27, 2011 · sputum can be stored at refrigerator temperature for five days, AFB remains in the sputum sample and DSSM can be done even after 3 days at room temperature but the odor becomes foul because of putrefaction. Sputum cups with a fixative are available in the Cytology Lab and Central Lab. Sputum specimens received without fixative will be rejected. Instruction to Patients for Sputum Specimen Collection 1. Label the sputum cup with patient’s name, hospital number and date of collection. 2. An early morning cough specimen gives the best yield of cells.
I am suffering from pulmonary TB. My doctor suggested a sputum test. I have a container for collecting sputum sample. Two days ago when I tired to collect a sample, only saliva came out in the container, but there was no sputum. Today while coughing I felt sputum in my mouth and I spitted in the same container . Now my concern is, will the sputum sample be useful even … A sputum fungal smear is a laboratory test that looks for fungus in a sputum sample. Sputum is the material that comes up from air passages when you cough deeply. Sputum fungal smear : MedlinePlus Medical Encyclopedia
Returning Your Sample You will need to return your sample to your clinic or health care provider who ordered the test: — the same day as collecting for the stool pathogen PCR panel — within 48 hours of collecting for other tests. Please bring a photo ID such as a driver’s license and your insurance card with you. Sep 11, 2017 · Sputum samples. Sputum samples can be obtained using a non-invasive or invasive method and ideally should be collected before antibiotics are started. Invasive methods include oropharyngeal or endotracheal suctioning; these are used with patients who are intubated. A sputum trap is connected to the suction catheter to collect the sputum (Fig 1) (Brekle, 2017).
Sputum Definition colors causes and when to see a doctor. Three-Day Sputum Sample What is a sputum sample? Sputum is the phlegm that you cough up from your lungs. Your doctor has asked for a sputum sample on three different days to help find out what may be causing your lung symptoms. It is best if you can do these sputum samples three days in a row. These samples will be done at home., Sep 11, 2017В В· Sputum samples. Sputum samples can be obtained using a non-invasive or invasive method and ideally should be collected before antibiotics are started. Invasive methods include oropharyngeal or endotracheal suctioning; these are used with patients who are intubated. A sputum trap is connected to the suction catheter to collect the sputum (Fig 1) (Brekle, 2017)..
Sputum sample strorage Connecting our members with. All Answers ( 15) It is recommended to refrigerate (do not freeze) sputum samples as soon as possible after collection to reduce multiplication of other bacteria (fast growers). The yield starts reducing by ten folds at 2-80c after 7 days. The earlier you process after collection the better. https://en.wikipedia.org/wiki/Pneumonia Collecting a Sputum Sample Your health care provider has ordered a other side is the one you will need to do. Before You Collect Your Sample You will be given the collection container(s) for your lab test before you are sent home. Write the following information on each of your collection containers: — your full name — date of collection.
Apr 23, 2008 · How Long Can I Keep A Urine Specimen Before I Take It To The Dr.and Does It Need To Be Refrigerated? I get back pain and then it will stop when I … I am suffering from pulmonary TB. My doctor suggested a sputum test. I have a container for collecting sputum sample. Two days ago when I tired to collect a sample, only saliva came out in the container, but there was no sputum. Today while coughing I felt sputum in my mouth and I spitted in the same container . Now my concern is, will the sputum sample be useful even …
Processing of sputum samples. Each sputum sample was split into two equal parts that were randomly allocated; one part was allocated to the standard method in which a nonrefrigerated centrifuge (Haereaus megafuge 1.0) was used, and the other was allocated to the modified method in which a refrigerated centrifuge (Beckman J-6B) was used. May 01, 2011В В· Can sputum be refrigerated? Question. It is common practice now to allow refrigeration of sputum samples prior to processing, in some cases up to 24 hours? Our lab does not allow sputum samples to be refrigerated because of the possibility of loss of possible Neisseria and Haemophilus species. Is refrigerating sputum samples acceptable practice?
Processing of sputum samples. Each sputum sample was split into two equal parts that were randomly allocated; one part was allocated to the standard method in which a nonrefrigerated centrifuge (Haereaus megafuge 1.0) was used, and the other was allocated to the modified method in which a refrigerated centrifuge (Beckman J-6B) was used. Dec 04, 2012 · Given the sensitivity of sputum culture especially with liquid systems (MGIT) over smear microscopy, one would imagine the incremental diagnostic yield of the EM sputum sample to be negligible; however, this is contrary to our findings which are in agreement with the previous studies by either microscopy or culture [7, 18–20].
The first step in the analysis of a fresh sputum sample is a Gram stain to identify the general type of bacteria that may be present and to determine sample adequacy. If a sample contains a significant number of normal cells that line the mouth (squamous epithelial cells), then the sample is not generally considered adequate for culture and a May 19, 2014В В· The stool sample container does not need to be brimming to the top with dog poop. It really only takes a little bit of poop. Usually, one gram of stool is plenty. DON'T bring in a sample that is old and all dried up. DO bring a fresh sample when you can. If you can't bring the sample to the doctor's office immediately, keep it in your refrigerator.
Running laboratory tests on sputum is the best way to find out if a patient has TB disease. Sputum results tell us if the TB medicine is working. III. How to do we collect sputum? The Public Health Investigator (PHI) will: 1. Instruct the patient on how to collect the sputum sample. 2. Hand an intact sputum container to the patient. Sep 11, 2017В В· Sputum samples. Sputum samples can be obtained using a non-invasive or invasive method and ideally should be collected before antibiotics are started. Invasive methods include oropharyngeal or endotracheal suctioning; these are used with patients who are intubated. A sputum trap is connected to the suction catheter to collect the sputum (Fig 1) (Brekle, 2017).
Children and women tend to swallow sputum. During sleep, there may be a tendency to swallow sputum. The object of the gastric lavage is to collect the accumulated sputum from the stomach. To do it right, you should awaken the patient in the morning and so a gastric lavage before anyone in the room has breakfast delivered. Jul 23, 2010В В· Answer. I think the best way to obtain a sputum sample is for your doctor to give you a sterile cup and let you produce one on your own time. If you're not producing one, he'll just have to wait. Keep in mind this answer was based on my own personal and professional opinion.
Returning Your Sample You will need to return your sample to your clinic or health care provider who ordered the test: — the same day as collecting for the stool pathogen PCR panel — within 48 hours of collecting for other tests. Please bring a photo ID such as a driver’s license and your insurance card with you. Sputum cups with a fixative are available in the Cytology Lab and Central Lab. Sputum specimens received without fixative will be rejected. Instruction to Patients for Sputum Specimen Collection 1. Label the sputum cup with patient’s name, hospital number and date of collection. 2. An early morning cough specimen gives the best yield of cells.
If the sputum sample is obtained in the evening the sample should be kept in the fridge overnight to try and keep the sample fresh. Taking a sputum sample at times when you do not have an infection e.g. when you attend the clinic for a regular appointment, is also helpful for deciding your long term treatment. May 01, 2011В В· Can sputum be refrigerated? Question. It is common practice now to allow refrigeration of sputum samples prior to processing, in some cases up to 24 hours? Our lab does not allow sputum samples to be refrigerated because of the possibility of loss of possible Neisseria and Haemophilus species. Is refrigerating sputum samples acceptable practice?
Dec 04, 2012 · Given the sensitivity of sputum culture especially with liquid systems (MGIT) over smear microscopy, one would imagine the incremental diagnostic yield of the EM sputum sample to be negligible; however, this is contrary to our findings which are in agreement with the previous studies by either microscopy or culture [7, 18–20]. Your doctor will probably need about 1 teaspoon (5 milliliters) of sputum to run the test. Try to spit out as much sputum and as little saliva as you can. There are no known risks to the test.
Can I put sputum sample in fridge? The advice here is fresh as possible, on the morning of handing it in for example, huff x. Content on HealthUnlocked does not replace the relationship between you and doctors or other healthcare professionals nor the advice you receive from them. *don't use more than 20% propylene glycol with water when inducing a sputum specimen in someone suspected of having tuberculosis; a higher concentration of propylene glycol inhibits growth of the pathogen and causes erroneous test results *use 10% to 20% acetylcysteine with water or sodium chloride if propylene glycol isn't available
*don't use more than 20% propylene glycol with water when inducing a sputum specimen in someone suspected of having tuberculosis; a higher concentration of propylene glycol inhibits growth of the pathogen and causes erroneous test results *use 10% to 20% acetylcysteine with water or sodium chloride if propylene glycol isn't available Method. Description. Advantage. Disadvantage. Spontaneous sputum sample. Patient coughs up sputum into a sterile container • Inexpensive • Easy to do • Patient may not be able to cough up sputum without assistance or may spit up saliva instead • Health-care worker has to coach and supervise the patient when collecting sputum Sputum Induction. Patient inhales a saline mist …